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Series GSE117961 Query DataSets for GSE117961
Status Public on Feb 25, 2019
Title RNA-seq datasets for "A neuron-optimized CRISPR/dCas9 activation system for robust and specific gene regulation"
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary This dataset contains whole-genome RNA sequencing results from rat embryonic hippocampal neuronal cultures and serves as the basis for characterization of CRISPR/dCas9 gene activation in neuronal systems.
 
Overall design This experiment contains 9 biological samples, each of which underwent directional, paired-end PolyA+ RNA-seq on an Illumina Next-seq 500. Samples were treated with Lacz sgRNA (LZ2, LZ4, & LZ5), Bdnf-I sgRNA (B16, B17, B18), or Bdnf-IV sgRNA (BIV11, BIV14, BIV15), in addition to a dCas9-VPR fusion. Datasets were obtained using RNA-seq from PolyA+ fractions fractions of RNA. Each sample has multiple files, corresponding to different sequencing lanes (e.g., L001, L002, etc) or different reads (e.g., R1, R2).
 
Contributor(s) Savell KE, Bach SV, Zipperly ME, Revanna JS, Goska NA, Tuscher JJ, Duke CG, Sultan FA, Burke JN, Williams D, Ianov L, Day JJ
Citation(s) 30863790
Submission date Jul 31, 2018
Last update date Mar 20, 2019
Contact name Jeremy Jason Day
E-mail(s) jjday@uab.edu
Phone 205-996-8960
Organization name UAB
Department Neurobiology
Lab Day
Street address 1825 University Blvd
City Birmingham
State/province AL
ZIP/Postal code 35294
Country USA
 
Platforms (1)
GPL20084 Illumina NextSeq 500 (Rattus norvegicus)
Samples (9)
GSM3315872 LZ2_S9
GSM3315873 LZ4_S10
GSM3315874 LZ5_S11
Relations
BioProject PRJNA483797
SRA SRP155892

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117961_BDNF_dCas9VPR_targeting.xlsx 7.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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