|
| Status |
Public on Dec 01, 2018 |
| Title |
RNA-seq reveals novel PAF1c regulation of transcription in mouse hematopoietic progenitor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Polymerase Associated Factor 1 complex (PAF1c) functions at the interface of epigenetics and gene transcription and plays a role in solid tumors and leukemia. Previous reports demonstrated that the PAF1c is required for MLL-fusion driven acute myeloid leukemia (AML) through the direct regulation of pro-leukemic target genes like Hoxa9 and Meis1. This is due to direct interactions between the PAF1c and wild type MLL or MLL fusion proteins. However, a detailed exploration of the PAF1c in normal hematopoiesis is lacking. Here, we utilize mouse genetic models to interrogate the role of the PAF1c subunit, Cdc73, in the development and maintenance of normal hematopoiesis. Using hematopoietic-specific expression of Cre recombinase, we discovered that Cdc73 is required for both embryonic and adult hematopoiesis. Here, we performed gene expression profiling (RNA-seq) in normal ckit+ HSPCs expressing Cdc73 or lacking Cdc73. Gene expression profiling in normal ckit+ HSPCs revealed differential regulation of Hoxa9 and Meis1 gene programs by CDC73 in normal hematopoietic cells compared to previously reported effects on AML cells.
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| Overall design |
Differential gene expression of genes regulated by Cdc73 in mouse hematopoietic cells. RNA-seq of cKit+ bone marrow from 3 mice with hematopoietic specific deletion of Cdc73 or 3 control mice performed on Illumina HiSeq4000
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| Contributor(s) |
Saha N, Ropa J, Muntean A |
| Citation(s) |
31031188 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL136420 |
The role of the PAFc subunit Cdc73 in normal hematopoiesis and transformation |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Andrew George Muntean |
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| |
| Submission date |
Jul 26, 2018 |
| Last update date |
Sep 18, 2019 |
| Contact name |
James Ropa |
| E-mail(s) |
jropa@iu.edu
|
| Organization name |
Indiana University
|
| Street address |
950 W Walnut St
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA483063 |
| SRA |
SRP155417 |
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