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| Status |
Public on Jul 26, 2018 |
| Title |
Gene expression profile of DLBCL cells emerged in Cg1-Cre Spi1 KO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-seq analysis and compared the differences in gene expression between tumor cells from Cγ1-Cre Spi1 KO mice and B cells from PU.1-loxP mice.
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| Overall design |
We performed RNA-seq analysis of tumor cells from Cγ1-Cre Spi1 KO mice and B cells from PU.1-loxP mice. We analyzed DLBCL cells from three Cγ1-Cre Spi1 KO mice and splenic B cells from two Cγ1-Cre Spi1 KO mice with mild splenomegaly. We also analyzed B cells from spleen of two PU.1-loxP mice. B220+ cells were purified using B220 magnetic beads (Miltenyi Biotec) and RNAs were extracted using Trizol reagent (Thermo Fisher Scientific) and RNeasy Mini Kit (Qiagen). We next compared the differences in gene expression between tumor cells from Cγ1-Cre Spi1 KO mice and those from Spi1-loxP mice.
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| Contributor(s) |
Endo S, Nishimura N, Yuki H, Masao M, Okuno Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 25, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Yutaka Okuno |
| E-mail(s) |
yokuno@gpo.kumamoto-u.ac.jp
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| Phone |
+81-96-373-5156
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| Organization name |
Kumamoto university of Medicine
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| Department |
Department of Hematology
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| Street address |
1-1-1 Honko, Chuo Ku
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| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA482758 |
| SRA |
SRP155150 |
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