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| Status |
Public on Aug 02, 2019 |
| Title |
Long non-coding RNA SMILR regulates genes involved in cytokinesis in human vascular smooth muscle cell |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In response to blood vessel wall injury, aberrant proliferation of vascular smooth muscle cells causes pathological remodelling. The controlling mechanisms involved are, however, not completely understood. We recently showed that the intergenic human, poorly-conserved long non-coding RNA, SMILR, promotes vSMC proliferation. Here, using deep RNA-sequencing of vSMCs with SMILR knockdown or overexpression, we identified an interconnected cell cycle network controlled by SMILR. SMILR inhibition led to vSMC binucleation and mitotic arrest.
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| Overall design |
RNA sequencing was performed on primary Human Saphenous Vein Smooth Muscle Cells (HSVSMCs), stimulated with IL1a (10 ng/mL) and PDGF-ββ (20 ng/mL), exposed to either SMILR depletion via siRNA (siSMILR) or overexpression via lentivirus (SMILR_LV). Respective control corresponds to siCONTROL or empty lentivirus. Each treatment was done in triplicates.
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| Contributor(s) |
Mahmoud AD, Ballantyne MD, Rodor J, Baker AH |
| Citation(s) |
31339449 |
| |
| Submission date |
Jul 24, 2018 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Julie Rodor |
| E-mail(s) |
Julie.Rodor@ed.ac.uk
|
| Phone |
+44 (0)7580200884
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| Organization name |
UNIVERSITY OF EDINBURGH
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| Department |
Centre for Cardiovascular Science
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| Street address |
47 Little France Crescent
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| City |
Edinburgh |
| State/province |
NA |
| ZIP/Postal code |
EH16 4TJ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA482638 |
| SRA |
SRP155025 |
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