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| Status |
Public on Dec 25, 2023 |
| Title |
PRMT5 knockdown changes gene expression in prostate cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
PRMT5 is the major type II protein arginine methyltransferase catalyzing the symmetric dimethylation of arginine. Recent reports have indicated that PRMT5 is overexpressed in multiple cancer types, including prostate. However, the exact contribution of PRMT5 to prostate tumorigenesis is unknown. To explore the functional role of PRMT5 in prostate cancer (PCa), we knocked down PRMT5 by lentiviral shRNAs in both AR-dependent LNCaP cells and AR-independent PC3 cells. Our data suggests that PRMT5 regulates PCa cell biology. To further identify PRMT5-regulated genes in PCa, we performed paired-end RNA-seq analysis in PC3 and LNCaP cells with or without PRMT5-knockdown.
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| |
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| Overall design |
Quantification of RNA expression in prostate PC3 and LNCaP cancer cells infected with shNC lentivirus or PRMT5-targeting shRNAs to inhibit PRMT5 signaling.
|
| Web link |
https://www.sciencedirect.com/science/article/pii/S0304383524006098
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| |
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| Contributor(s) |
Zhang D, Hu Q, Tang DG |
| Citation(s) |
39218291 |
| |
| Submission date |
Jul 24, 2018 |
| Last update date |
Sep 03, 2024 |
| Contact name |
Dingxiao Zhang |
| E-mail(s) |
zdx1980@hnu.edu.cn
|
| Phone |
+86-15391542415
|
| Organization name |
Hunan University
|
| Department |
School of BioMedical Sciences
|
| Lab |
Zhang lab
|
| Street address |
Yuelushan
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410082 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA482621 |
| SRA |
SRP155003 |
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