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| Status |
Public on Jul 24, 2018 |
| Title |
RNA-sequencing of liver samples derived from offspring sired by males chronically exposed to alcohol prior to conception |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using an established mouse model of chronic exposure, previous work by our group had linked preconception paternal alcohol use to sex-specific patterns of fetal growth restriction and placental dysfunction. The goal of the present study was to investigate the long-term impact chronic preconception paternal alcohol exposure has on offspring growth and metabolic programming. In the male offspring, we observed increases in both the expression of genes within the pro-fibrotic TGF-ß signaling pathway as well as total levels of cellular hydroxyproline within the liver. Further, we identified suppressed cytokine profiles, which could be linked to the up-regulation of genes in the LiverX/RetinoidX/FarnesoidX Receptor pathways.
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| Overall design |
Postnatal day 90 male mice were provided access to either a solution of 10% (w⁄v) alcohol and 0.066% (w⁄v) Sweet ‘N Low (experimental) or 0.066% (w⁄v) Sweet ‘N Low alone (control) for four hours a day. Once consistent patterns of drinking were established, males were maintained on this protocol for a period of 70 days, which corresponds to the length of approximately two complete spermatogenic cycles. Once the 70-day milestone was surpassed, males were mated to unexposed dams and the offspring evaluated for growth.
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| Contributor(s) |
Golding M, Chang RC |
| Citation(s) |
30670059 |
| |
| Submission date |
Jul 23, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
MICHAEL C. GOLDING |
| E-mail(s) |
mgolding@cvm.tamu.edu
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| Phone |
979-862-1332
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| Organization name |
Texas A&M UNiversity
|
| Department |
Physiology
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| Lab |
Golding Lab
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| Street address |
4466 TAMU
|
| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77843-4466 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA482462 |
| SRA |
SRP154937 |
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