|
| Status |
Public on Jan 07, 2019 |
| Title |
RNA sequencing of mouse epidermis and papillomas treated with 20% camphor white oil or acetone vehicle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Camphor white oil (CWO) application was found to induce regression of papillomas in a mouse chemical carcinogenesis model. In healthy epidermis, CWO induced increased proliferation of basal keratinocytes. RNA-sequencing was performed on epidermal cells 24 hours after CWO or vehicle application (N=3 mice/treatment), and on 4 pooled papillomas harvested after 6 weeks of treatment (N=3 mice/treatment). Sequencing identified transcripts that may alter skin physiology and induce tumor regression.
|
| |
|
| Overall design |
Examination of 2 tissue types treated with camphor white oil or vehicle.
|
| |
|
| Contributor(s) |
Moayedi Y, Greenberg SA, Lumpkin EA |
| Citation(s) |
30582219 |
| |
| Submission date |
Jul 23, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Ellen A Lumpkin |
| E-mail(s) |
eal2166@columbia.edu
|
| Phone |
2128514830
|
| Organization name |
Columbia University
|
| Department |
Physiology and Cellular Biophysics
|
| Street address |
1150 St. Nicholas Avenue, room 302
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA482457 |
| SRA |
SRP154936 |
Less...
More...