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| Status |
Public on Feb 25, 2019 |
| Title |
Fisetin induces autophagy in pancreatic cancer cells via endoplasmic reticulum stress- and mitochondrial stress-dependent pathways |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pancreatic cancer is one of the most aggressive tumors and has poor survival rates. Fisetin, a natural flavonoid, was recently reported to have antitumor effects in various cancer models. Autophagy is a conserved catabolic process that maintains cellular homoeostasis in response to stress, and together with apoptosis, determines cell fate. Herein, we examined the effect of fisetin on pancreatic cancer. We reveal that fisetin inhibits Panc-1 cell proliferation in vivo and in vitro. We found that AMPK-dependent autophagy was enhanced after fisetin treatment.However RNA-seq analysis revealed that the unfolded protein response, which is activated by ER stress, was enriched. We also found that there may be crosstalk between the AMPK- and p8-dependent pathways.
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| Overall design |
Total mRNA profiles of human pancreatic cancer Panc-1 cells with or without treatment of Fisetin, were generated by deep sequencing, in triplicate.
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| Contributor(s) |
Jia S, Xu X, Zhou S, Chen Y, Ding G, Cao L |
| Citation(s) |
30760707, 37743465 |
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| Submission date |
Jul 16, 2018 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Xiao Dong Xu |
| E-mail(s) |
xxd94@foxmail.com
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| Phone |
15757119342
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| Organization name |
Department of General Surgery,Sir Run Run Shaw Hospital,School of Medicine,Zhejiang University
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| Street address |
Administrative Building,3 East Qingchun Road, Hangzhou, Zhejiang, China.
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| City |
Hangzhou |
| State/province |
Zhejiang province |
| ZIP/Postal code |
310000 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA481372 |
| SRA |
SRP153992 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE117189_RAW.tar |
1.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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