|
| Status |
Public on Apr 07, 2019 |
| Title |
Physiological and molecular dissection of daily variance in exercise capacity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Physical performance relies on the concerted action of myriad responses, many of which are under circadian clock control. Little is known, however, regarding the time-dependent effect on exercise performance at the molecular level. We found that both mice and humans exhibit day-time variance in exercise capacity between the early and late part of their active phase. The day-time variance in mice was dependent on exercise intensity and relied on the circadian clock proteins PER1/2. High throughput gene expression and metabolic profiling of skeletal muscle revealed metabolic pathways that are differently activated upon exercise in a day-time dependent manner. Remarkably, we discovered that ZMP, an endogenous AMPK activator, is induced by exercise in a time-dependent manner to regulate key steps in glycolytic and fatty acid oxidation pathways and potentially enhance exercise capacity. Overall, we propose that time of the day is a major modifier of exercise capacity and associated metabolic pathways.
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| |
|
| Overall design |
basal, high intensity and moderate intensity runnig protocol at ZT14 and ZT22 in gastrocnemius muscle in C57B6 mice
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| |
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| Contributor(s) |
Ezagouri S, Zwighaft Z, Sobel J, Asher G |
| Citation(s) |
31006590, 33598660 |
| |
| Submission date |
Jul 16, 2018 |
| Last update date |
Feb 23, 2021 |
| Contact name |
Jonathan Aryeh Sobel |
| E-mail(s) |
jonathan.sobel@weizmann.ac.il
|
| Phone |
00972587889058
|
| Organization name |
Weizmann Institute of science
|
| Department |
Biomolecular Sciences
|
| Lab |
Asher
|
| Street address |
Benoziyo Building for Biological Sciences, room 277S, Weizmann Institute of Science
|
| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA481324 |
| SRA |
SRP153906 |
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