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| Status |
Public on Jul 17, 2018 |
| Title |
RNA-seq after siRNA targeting DDX24 applied to iHUVECs cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
DDX24 Mutations Associated with Vascular Malformations
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| Overall design |
To detect the vascular-related functions of the causative gene, two short interfering (si)RNAs targeting DDX24 were designed by GenScript bioinformatics tools (https://www.genscript.com/tools/sirna-target-finder) and transfected into immortal human umbilical vein endothelial cells (iHUVECs). A vehicle comprising scrambled siRNA (RIBOBIO) was used as a control. The knockdown efficiency was determined by real-time quantitative PCR and western blotting. RNA sequencing analysis was performed using the siRNA-targeted HUVEC cells to evaluate the effects of the causative gene knockdown on gene expression. Genes involved in cell migration were validated by real-time quantitative PCR.
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| |
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| Contributor(s) |
Pang P, Hu X, Zhou B, Mao J, He H, Shan H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 16, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Pengfei Pang |
| E-mail(s) |
huxiaoj5@mail.sysu.edu.cn
|
| Organization name |
The Third Affiliated Hospital,Sun Yat-Sen University
|
| Street address |
No. 600 Tianhe Road, Tianhe District
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA481255 |
| SRA |
SRP153815 |
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