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| Status |
Public on Mar 21, 2019 |
| Title |
Long noncoding RNA signatures induced by TLR7 and type I IFN signaling in activated human plasmacytoid dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using genome-wide sequencing approaches we viewed the contributions of Toll-like receptor 7 (TLR7) and type I interferon (IFN-I) in the regulation of coding and noncoding RNA expression in CAL-1 pDC treated with R848 or IFNβ. Functional enrichment analysis revealed both the unique and synergistic roles of TLR7 and IFN-I signaling in the orchestration of pDC function.
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| Overall design |
Total RNA-seq profiling was performed on CAL-1 cells (1 million cells/mL) were cultured in 0.1% FBS CAL-1 media in triplicate in a 12- well tissue culture plate for 6 hours prior to stimulation with 1,000 U/mL recombinant human IFN-β (PBL Interferon Source) or 1ug/mL R848 (Invitrogen) or 1ug/mL R848 (Invitrogen) + 1ug/mL recombinant vaccinia virus B18R protein (Thermo Fisher Scientific) or mock stimulation. Total RNA was harvested 12 hours post stimulation
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| Contributor(s) |
Joslyn R, Forero A, Ozarkar S, Lee CH, So L, Green R, Savan R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 16, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Michael Gale, Jr |
| E-mail(s) |
uw_galelab_geo@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Immunology
|
| Street address |
750 Republican St. E360, Box 358059
|
| City |
Seattle |
| State/province |
Washington |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| GSM3271607 |
CAL-1 control mock treated at 12hr rep 1 |
| GSM3271608 |
CAL-1 control mock treated at 12hr rep 2 |
| GSM3271609 |
CAL-1 control mock treated at 12hr rep 3 |
| GSM3271610 |
CAL-1 control IFN Beta treated at 12hr rep 1 |
| GSM3271611 |
CAL-1 control IFN Beta treated at 12hr rep 2 |
| GSM3271612 |
CAL-1 control IFN Beta treated at 12hr rep 3 |
| GSM3271613 |
CAL-1 control IFN Lambda treated at 12hr rep 1 |
| GSM3271614 |
CAL-1 control IFN Lambda treated at 12hr rep 2 |
| GSM3271615 |
CAL-1 control IFN Lambda treated at 12hr rep 3 |
| GSM3271616 |
CAL-1 control IFN Beta treated at 12hr rep 1 with R848 + recombinant vaccinia virus B18R protein |
| GSM3271617 |
CAL-1 control IFN Beta treated at 12hr rep 2 with R848 + recombinant vaccinia virus B18R protein |
| GSM3271618 |
CAL-1 control IFN Beta treated at 12hr rep 3 with R848 + recombinant vaccinia virus B18R protein |
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| Relations |
| BioProject |
PRJNA481248 |
| SRA |
SRP153809 |
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