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| Status |
Public on Sep 07, 2018 |
| Title |
RNA-seq analysis comparing gene expression in Drosophila sea mutants and controls |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to determine how decreased mitochondrial citrate export influences gene expression in Drosophila larvae. RNA was isolated from Drosopohila sea mutants, which exhibiti decreased mitochondrial citrate transport activity, and a genetically-matched control strain during mid-L3 development.
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| Overall design |
Larvae were collected as described in Li, H., Tennessen, J. M. Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics. J. Vis. Exp. (136), e57847, doi:10.3791/57847 (2018). RNA was purified from staged mid-L3 larvae using a RNeasy Mini Kit (Qiagen). Sequencing was performed using an Illumina NextSeq500 platform with 75 bp sequencing module generating 41 bp paired-end reads. After the sequencing run, demultiplexing was performed with bcl2fastq v2.20.0.422.
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| Contributor(s) |
Li H, Tennessen J |
| Citation(s) |
30108060 |
| |
| Submission date |
Jul 13, 2018 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Jason Tennessen |
| E-mail(s) |
jtenness@indiana.edu
|
| Phone |
812 855 9803
|
| Organization name |
Indiana University
|
| Department |
Department of Biology
|
| Street address |
1001 E. Third St
|
| City |
Bloomington |
| State/province |
IN |
| ZIP/Postal code |
47405 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA481071 |
| SRA |
SRP153550 |
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