|
| Status |
Public on Jul 13, 2018 |
| Title |
Cancer associated mutants of eIF1A impair Rps3/Rps10 binding and enhance scanning of cell cycle genes [Mars-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The Ribo-seq analysis demonstrated that eIF1A is predominantly essential for translation of genes with long 5'UTR genes including cell proliferation and cell cycle progression genes. eIF1A depletion causes broad stimulation of initiation in 5’UTRs at near-cognate AUG codons that diminshes the translation initiation fidelity
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| |
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| Overall design |
Mars-seq analysis was performed either in contol or eIF1A knockdown MEFs
|
| |
|
| Contributor(s) |
Sehrawat U, Ashkanazi S, Koning F, Stelzer G, Leshkowitz D, Dikstein R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 12, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Dena Leshkowitz |
| E-mail(s) |
dena.leshkowitz@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Bioinformatics Unit, Life Sciences Core Facilities
|
| Street address |
P.O.B. 26
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE116983 |
Cancer associated mutants of eIF1A impair Rps3/Rps10 binding and enhance scanning of cell cycle genes |
|
| Relations |
| BioProject |
PRJNA480788 |
| SRA |
SRP153151 |
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