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| Status |
Public on Jul 06, 2022 |
| Title |
Comparison of the Gr-1+ and Gr-1- monocyte gene expression in cell-type specific Fcgr2 deficiency on the Yaa backgound |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We generated 5 mouse lines:(1) Fcgr2 -/-.Yaa,(2)CD19Cre;Fcgr2 fl/fl.Yaa, (3)CEBPaCre;Fcgr2 fl/fl.Yaa, (4)CD11cCre;Fcgr2 fl/fl.Yaa, and (5)Fcgr2 fl/fl.Yaa. Peripheral blood mononuclear cells from each mouse at 8 months old were collected and sorted by CD11b and Gr-1 cell surface markers. Sorted cells were subjected to RNA extraction and RNA-seq analysis
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| |
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| Overall design |
Either Gr-1+CD11b+ or Gr-1-CD11b+ cell mRNA profiles of various 8 month-old mice were generated by deep sequencing using HiSeq 1500 sequencer (Illumina)
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| Contributor(s) |
Fukuyama H, Sato R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 07, 2018 |
| Last update date |
Jul 08, 2022 |
| Contact name |
Hidehiro FUKUYAMA |
| E-mail(s) |
hidehiro.fukuyama@riken.jp
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Lymphocyte Differentiation
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| Street address |
1-7-22, Suehirocho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA480069 |
| SRA |
SRP152622 |
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