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Status |
Public on Jul 06, 2018 |
Title |
RNA-seq of eight lymphoblastoid cell lines after AHR ligand treatments |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
RNA-seq was done on eight different lymphoblastoid cell lines for vehicle control, 3-MC agonist treatment and GNF-351 antagonist treatment for AHR to look at AHR regulated genes with different genetic backgrounds. We identified 69 genes that were highly differentially expressed after 3-MC or GNF-351 antagonist treatment with variation in the degree fo differential expression between the eight cell lines.
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Overall design |
Eight lymphoblastoid cells lines were treated in duplicate with vehicle control, 3-MC or GNF-351 for 24 hours before RNA collection. Libraries were prepared on the TruSeq RNA Library Prep Kit v2 and paired end sequencing was carried out on Illumina HiSeq 4000
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Contributor(s) |
Weinshlboum RM, Neavin DR, Wang L |
Citation(s) |
31292129 |
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Submission date |
Jul 05, 2018 |
Last update date |
May 12, 2020 |
Contact name |
Drew Neavin |
E-mail(s) |
d.neavin@garvan.org.au
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Organization name |
Garvan Institute of Medical Research
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Department |
Cellular Science
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Street address |
384 Victoria St
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City |
Darlinghurst |
State/province |
New South Wales |
ZIP/Postal code |
2010 |
Country |
Australia |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (48)
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This SubSeries is part of SuperSeries: |
GSE116638 |
Role of AHR in lymphoblastoid cell lines |
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Relations |
BioProject |
PRJNA479764 |
SRA |
SRP152203 |