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| Status |
Public on Jun 01, 2020 |
| Title |
High-throughput sequencing reveals induction of drug metabolism in various cholestatic livers |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cholestasis is a multifactorial disorder that compromises liver function. We wanted to examine Phase I and II drug metabolism in cholestatic livers. We performed high-throughput sequencing on livers from Fxr-/- Shp-/- double knockout mice, which mimic juvenile cholestasis. Next, we used comparative transcriptomic approach to test if the induction in drug metabolism is also seen in models of liver injury, including steatosis, regeneration and bile acid-stressed livers.
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| Overall design |
Hepatic mRNA expression profiles of adult wild type, CAR-activated (TCPOBOP injected) (n=2), Fxr-/- Shp-/- (n=3), high fat diet-fed (n=2), partial hepatectomized (n=3) and bile acid diet-fed (n=3) mice.
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| Contributor(s) |
Mathur B, Arif W, Patton ME, Rahiman F, Kalsotra A, Wheatley A, Anakk S |
| Citation(s) |
32875282 |
| |
| Submission date |
Jul 04, 2018 |
| Last update date |
Sep 08, 2020 |
| Contact name |
Waqar Arif |
| E-mail(s) |
warif2@illinois.edu
|
| Organization name |
UIUC
|
| Department |
Biochemistry
|
| Lab |
461 Medical School Building
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| Street address |
506 S. Mathews Avenue
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA479694 |
| SRA |
SRP152043 |
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