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| Status |
Public on Feb 12, 2019 |
| Title |
Gene expression analysis of WT and Stat1-/- CD4+ T cells pre and post transfer into Rag1-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the basis behind the defective survival/expansion of Stat1-/- CD4+ T cells in Rag1-/- mice, we analyzed the gene expression of WT and Stat1-/- CD4+ T cells pre- and post-transfer into these mice by RNAseq.
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| Overall design |
WT (C57BL/6) or Stat1-/- CD4+ T cells (3 donor mice/group) were MACS sorted by negative selection. Some of the T cells from each mouse was used for RNAseq (Pre-transfer, WT=1, KO=2). The remaining T cells were injected into Rag1-/- mice (5 mice/group) and FACS sorted from the spleens and lymph nodes (CD45+ CD3ε+ CD4+) 7 days later. In the post-transfer setting, the T cells from the 5 mice of each group were split into 3 biological replicates each containing 1-2 mice (denoted as WT or KO).
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| Contributor(s) |
Kang Y, Biswas A, Field M, Snapper SB |
| Citation(s) |
30796216 |
| |
| Submission date |
Jul 01, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Scott Snapper |
| E-mail(s) |
scott.snapper@childrens.harvard.edu
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| Organization name |
Boston Children's Hospital
|
| Department |
GI/Nutrition
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| Street address |
300 Longwood Avenue, Enders 670
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA478772 |
| SRA |
SRP151712 |
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