|
| Status |
Public on Nov 15, 2018 |
| Title |
RNA-seq of synchronized S phase or G2 phase cells treated with an ATR inhibitor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNA-seq in cells synchronized to S or G2 phase to identify genes that were transcriptionally regulated by ATR activity.
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| |
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| Overall design |
RPE-1 cells were synchronized to late S (4 hours post-release from thymidine block), or to G2 phase (6 hours post-released from thymidine block) and either mock-treated or ATR-inhibited to identify genes that were transcriptionally controlled by ATR. An asynchronous control and an un-released from the thymidine block control were included. These 6 conditions were repeated in 3 independent experiments (total of 18 samples) and analyzed by RNA-seq.
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| |
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| Contributor(s) |
Bocek M, Saldivar J, Cimprich K, Hamperl S |
| Citation(s) |
30139873 |
| |
| Submission date |
Jun 21, 2018 |
| Last update date |
Jan 14, 2020 |
| Contact name |
Karlene Anne Cimprich |
| E-mail(s) |
cimprich@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
318 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94025 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA477351 |
| SRA |
SRP151048 |
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