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Series GSE116015 Query DataSets for GSE116015
Status Public on Aug 20, 2019
Title Organoid-induced differentiation of conventional T cells from human pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Generation of T cells from pluripotent stem cells (PSC) has the potential to transform adoptive immunotherapy for cancer into universal donor, off-the-shelf cellular therapies. However, differentiation of human PSCs into fully mature T cells has been challenging with existing methods. We report that a 3D organoid method permitted efficient differentiation of human embryonic stem cell and induced pluripotent stem cell-derived mesoderm progenitors to mature, functional conventional T cells with a diverse T cell receptor (TCR) repertoire. This continuous culture system supported both hematopoietic induction and terminal differentiation to naïve, conventional CD3+CD8αβ+ and CD3+CD4+ T cells. Introduction of a Class I-restricted TCR in PSCs produced antigen-specific CD8αβ+ T cells lacking endogenous TCR expression. Functional assays and RNA sequencing aligned PSC-derived T cells with primary naïve conventional CD8+ T cells. The organoid system presented here provides an effective and scalable platform to generate functional mature T cells from human PSCs.
Overall design Our goal was to analyze and compare transcriptome of human naïve T cells generated in Artificial Thymic Organoids (ATOs) from PSC with human naïve T cells from thymus and Cord blood ATOs.
Contributor(s) Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Lopez S, Tsai S, He CB, Chin CJ, Casero D, Crooks GM
Citation(s) 30661959
Submission date Jun 19, 2018
Last update date Aug 20, 2019
Contact name Gay Miriam Crooks
Phone 3102060205
Organization name University of California Los Angeles
Department Pathology & Laboratory Med
Street address 610 Charles E. Young Drive, East
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (32)
GSM3207149 PSC (H1 ESC) sample 1
GSM3207150 PSC (H1 ESC) sample 2
GSM3207151 EMP sample 1
BioProject PRJNA476793
SRA SRP150879

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Supplementary file Size Download File type/resource
GSE116015_GCAMhsExp.GEO.txt.gz 1.9 Mb (ftp)(http) TXT
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Processed data are available on Series record

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