|
| Status |
Public on Feb 13, 2019 |
| Title |
Simultaneous quantification of antibody-RNA conjugates and the transcriptome by single cell RNA-sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Undifferentiated and differentiated Keratinocytes (AG1478 treated) were stained with antibody-RNA conjugates (targeting EGFR and ITGA6) to measure protein-based differentiation changes in conjunction with single-cell transcriptomics.
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| |
|
| Overall design |
Two 384 wells plates for untreated and two 384 wells plates for AG1478 treated cells were processed for single cell transcriptomics.
|
| |
|
| Contributor(s) |
Gerlach JP, Mulder KW |
| Citation(s) |
30728416 |
| |
| Submission date |
Jun 18, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Jan Patrick Gerlach |
| E-mail(s) |
janpgerlach@gmail.com
|
| Organization name |
Radboud Institute for Molecular Life Sciences
|
| Department |
Molecular Developmental Biology
|
| Street address |
Geert Grooteplein Zuid 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6500HB |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE115981 |
RAID allows multiplexed quantification of intracellular (phospho-)proteins and transcriptomes from fixed single cells |
|
| Relations |
| BioProject |
PRJNA476555 |
| SRA |
SRP150758 |
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