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| Status |
Public on Feb 13, 2019 |
| Title |
Comparison of single-cell transcriptomics quality between unfixed cells and cells that were fixed and mock stained according to the RAID procedure |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cell fixation, permeabilization and antibody staining of could have adverse effects on the quality of single cell transcriptomics data. To assess the effects of the RAID procedure, which includes such steps, we performed a direct comparison of single cell transcriptomics by CELseq2 using unfixed and RAID-processed cells. Quality measures (gene complexity, gene detection rate, average gene expression) were performed using 40000 samples UMI counts per cell.
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| Overall design |
Single cells were sorted in 96, wells plates. Per condition (unfixed or RAID) three sets (A,B,C) of 48 cells were processed with the CELseq2 protocol.
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| Contributor(s) |
Gerlach JP, Mulder KW |
| Citation(s) |
30728416 |
| |
| Submission date |
Jun 15, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Jan Patrick Gerlach |
| E-mail(s) |
janpgerlach@gmail.com
|
| Organization name |
Radboud Institute for Molecular Life Sciences
|
| Department |
Molecular Developmental Biology
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| Street address |
Geert Grooteplein Zuid 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6500HB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE115981 |
RAID allows multiplexed quantification of intracellular (phospho-)proteins and transcriptomes from fixed single cells |
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| Relations |
| BioProject |
PRJNA476320 |
| SRA |
SRP150624 |
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