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| Status |
Public on Jul 18, 2018 |
| Title |
Transcriptional profiling of mouse plasma cells (population RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CD138-high plasma cell subsets were analyzed by RNA-sequencing to identify gene expression differences between long- and short-lived plasma cells. Five different CD138-high subsets were analyzed based upon differential B220 expression and uptake of the fluorescent glucose analog 2NBDG: splenic B220+2NBDG-, B220+2NBDG+, B220-2NBDG-, B220-2NBDG+, and bone marrow B220-2NBDG+ cells.
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| Overall design |
Approximately 30,000 cells were analyzed of each subset. Three biological replicates of each subset were included. Samples were double sorted from 8-12 week old C57Bl6/N male mice.
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| Contributor(s) |
Bhattacharya D, Lam WY |
| Citation(s) |
30157439 |
| |
| Submission date |
Jun 15, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Deepta Bhattacharya |
| Organization name |
University of Arizona
|
| Department |
Department of Immunobiology
|
| Street address |
1656 E. Mabel Street, MRB 217
|
| City |
Tucson |
| State/province |
Arizona |
| ZIP/Postal code |
85724 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE115860 |
Transcriptional profiling of mouse plasma cells |
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| Relations |
| BioProject |
PRJNA476324 |
| SRA |
SRP150625 |
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