|
| Status |
Public on Aug 20, 2018 |
| Title |
MicroRNA Regulation in Blood Cells of Renal Transplanted Patients with Interstitial Fibrosis/Tubular Atrophy and Antibody-Mediated Rejection |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We performed small RNA-Seq and compared expression levels of microRNAs of whole blood cells isolated from patients after kidney transplantation with stable graft function (SGF), antibody-mediated rejection (ABMR) and T cell-mediated rejection (TCMR).
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| |
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| Overall design |
Whole blood cells were isolated from 6 patients with stable graft function, 6 patients with histologically verified antibody-mediated graft rejection episode and 4 patients with histologically verified T cell-mediated graft rejection after kidney transplantation. Total RNA was extracted and cDNA libraries for small RNA sequencing were generated using "TruSeq® small RNA library Prep" kit (Illumina, San Diego, CA, USA).
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| |
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| Contributor(s) |
Matz M, Heinrich F, Lorkowski C, Wu K, Zhang Q, Lachmann N, Durek P, Budde K, Mashreghi MF |
| Citation(s) |
34206682 |
| |
| Submission date |
Jun 14, 2018 |
| Last update date |
Jul 14, 2021 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
|
| Organization name |
Deutsches Rheuma-Forschungszentrum
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (16)
|
| GSM3190422 |
Patients with antibody mediated rejection [S1] |
| GSM3190423 |
Patients with antibody mediated rejection [S2] |
| GSM3190424 |
Patients with antibody mediated rejection [S3] |
| GSM3190425 |
Patients with antibody mediated rejection [S4] |
| GSM3190426 |
Patients with antibody mediated rejection [S5] |
| GSM3190427 |
Patients with antibody mediated rejection [S6] |
| GSM3190428 |
Patients with stable graft function [S7] |
| GSM3190429 |
Patients with stable graft function [S8] |
| GSM3190430 |
Patients with stable graft function [S9] |
| GSM3190431 |
Patients with stable graft function [S10] |
| GSM3190432 |
Patients with stable graft function [S11] |
| GSM3190433 |
Patients with stable graft function [S12] |
| GSM3190434 |
Patients with T cell mediated rejection [S13] |
| GSM3190435 |
Patients with T cell mediated rejection [S14] |
| GSM3190436 |
Patients with T cell mediated rejection [S15] |
| GSM3190437 |
Patients with T cell mediated rejection [S16] |
|
| Relations |
| BioProject |
PRJNA476126 |
| SRA |
SRP150540 |
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