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| Status |
Public on Sep 01, 2018 |
| Title |
Ubiquitin-specific protease USP34 controls osteogenic differentiation and bone formation by regulating BMP2 signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim is to analyze the transcriptome profiling (RNA-seq) of Mettl3 knockout mesenchymal stem cells (MSCs) and identify m6A modified mRNThe aim is to analyze the transcriptome profiling (RNA-seq) of USP34 knockdown mesenchymal stem cells (MSCs). Total RNAs from human MSCs of siCTRL and siUSP34 were extracted using an RNeasy mini kit (Qiagen). Libraries were prepared using the Illumina TrueSeq mRNA sample preparation kit according to manufacturer’s instruction, and single-end sequenced on an Illumina HiSeq 3000 machine. The reads were aligned to hg19 genome using Tophat. Analysis of RNA-seq data was done using Cufflinks with Refseq mRNAs to guide assembly and the cummeRbund package in R. Transcripts, which were regulated greater than 2-fold were considered significant difference.
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| Overall design |
The mRNA profiles of siCTRL and siUSP34 MSCs were generated by deep sequencing, in duplicate, using Illumina HiSeq 3000.
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| Contributor(s) |
Yuan Q |
| Citation(s) |
30181118 |
| Submission date |
Jun 14, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
Quan Yuan |
| E-mail(s) |
yuanquan@scu.edu.cn
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| Organization name |
West China Hospital of Stomatology, Sichuan University
|
| Lab |
State Key laboratory of Oral Diseases
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| Street address |
14 Third section, Renmin Nan Road
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA476060 |
| SRA |
SRP150508 |
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