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| Status |
Public on Jul 01, 2020 |
| Title |
Enhancer-promoter association determines cell specificity in mouse pluripotent cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Enhancer-promoter association is emerging as a common pattern to determine gene function in eukaryotes. However, it remains as an uncultivated land on how its diversity influences gene expression. In this study, we select three defined Sox2 associated enhancers, representing different distal interaction categories, to further explore its biological effects. We construct three enhancer-knock-out cells via CRISPR/Cas9 system in mouse embryonic stem cells. With multiple-level evidences, we realize these associations carried out various biological features. Embryonic cell specific association determines cell proliferation and cell cycle and also involves in cardiac function, which has been validated in vivo. On the other hand, the common or indirect association prefers to regulate cell metabolic process and nerve development in cell function. Our study will enable an alternative way for gene spatiotemporal regulation study and advance our understanding of its diversity since first described decades ago.
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| Overall design |
Analysis of the effect of different promoter-enhancer associations on Sox2 diverse function in mouse embryonic stem cells
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| |
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| Contributor(s) |
Huang L, Li Q, Huang Q, Kong S, Zhu X, Peng Y, Zhang Y |
| Citation missing |
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| |
| Submission date |
Jun 13, 2018 |
| Last update date |
Jul 02, 2020 |
| Contact name |
Qing Li |
| E-mail(s) |
liqing9102@126.com
|
| Organization name |
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences,
|
| Street address |
No 7,Pengfei Road,Dapeng District
|
| City |
Shenzhen |
| ZIP/Postal code |
518120 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA475949 |
| SRA |
SRP150432 |
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