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| Status |
Public on Aug 31, 2018 |
| Title |
Human antimicrobial cytotoxic T lymphocytes, defined by NK receptors and antimicrobial proteins, kill intracellular bacteria |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human cytotoxic T cell subsets were sorted based on cytotoxic molecule expression and RNA sequencing was performed on these subsets
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| Overall design |
Human T cells from two donors were sorted by Flow based on expresssion of Granzyme B, Perforin and Granulysin in to subsets of cells experssing non of these markers, expressing ony granzyme B (monocytotoxic - MCTL) expressing granzyme B and perforin (dicytotoxic -DCTL), expressing all three (Tri-cytotoxic, TCTL - also called Polycytotoxic PCTL). RNA sequencing was performed on these sorted subsets and gene expression was compared to generate specific signatures of these subsets allowing for surface marker identification that permitted isolation for functional study.
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| |
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| Contributor(s) |
Balin SJ, Modlin RL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 12, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Samuel Jackson Balin |
| E-mail(s) |
sbalin@mednet.ucla.edu
|
| Phone |
3108256911
|
| Organization name |
UCLA
|
| Street address |
10833 Le Conte Ave, Div of Dermatology, 52-121 CHS
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-3075 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (11)
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| Relations |
| BioProject |
PRJNA475765 |
| SRA |
SRP150351 |
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