|
| Status |
Public on May 28, 2019 |
| Title |
STAT1, STAT2 and IRF9 transcription factor binding analysis in wild type and Irf9-/- bone marrow derived macrophages in response to type I and type II interferons |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Host defense by the innate immune system requires the establishment of antimicrobial states allowing cells to cope with microorganisms before the onset of the adaptive immune response. Interferons (IFN) are of vital importance in the establishment of cell-autonomous antimicrobial immunity. Speed is therefore an important attribute of the cellular response to IFN. With much of the antimicrobial response being installed de novo, this pertains foremost to gene expression, the rapid switch between resting-state and active-state transcription of host defense genes. Mechanisms to meet this demand on the relevant molecular machinery include remodeling of chromatin but also changes in transcription factor interaction prior and during the IFN response. Our results show how transcription factors STAT1, STAT2 and IRF9 change binding patterns upon IFNb or IFNg treatment in wild type and Irf9-/- bone marrow derived macrophages.
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| |
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| Overall design |
Methods: Genome wide binding of transcription factors STAT1, STAT2 and IRF9 in untreated, 90 min IFNb and 90 min IFNg treated wild-type (WT) and Irf9 knock out (IRF9-/-) bone marrow derived macrophages. Biological duplicates were analyzed by using Illumina sequencing.
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| |
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| Contributor(s) |
Platanitis E, Decker T |
| Citation(s) |
31266943, 35243225 |
|
https://doi.org/10.1101/377275
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| |
| Submission date |
Jun 06, 2018 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Ekaterini Platanitis |
| Organization name |
University of Vienna
|
| Department |
Department of Microbiology, Immunobiology and Genetics
|
| Street address |
Dr.-Bohr-Gasse 9
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (31)
|
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| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA474952 |
| SRA |
SRP149943 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE115433_K_STAT1_b_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
151.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_K_STAT1_g_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
163.3 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_K_STAT1_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
2.2 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_K_STAT2_b_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
274.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_K_STAT2_g_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
6.1 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_K_STAT2_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
2.5 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_RAW.tar |
39.4 Mb |
(http)(custom) |
TAR (of NARROWPEAK) |
| GSE115433_W_IRF9_b_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
317.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_IRF9_g_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
98.4 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_IRF9_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
13.0 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT1_b_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
159.5 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT1_g_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
171.0 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT1_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
1.8 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT2_b_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
376.6 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT2_g_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
86.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE115433_W_STAT2_ppr.peak.spp.idr.optimal_set.IDR0.01.filt.narrowPeak.gz |
14.8 Kb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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