|
| Status |
Public on Jan 11, 2019 |
| Title |
ZNF445 is a primary regulator of genomic imprinting |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ZFP445/ZNF445 collaborates with ZFP57 to control genomic imprinting in vivo in mouse and in human embryonic stem cells. We determined that ZNF445/ZFP445, a KZFP that can be traced back to marsupials, binds human and murine ICRs, and together with ZFP57 correctly maintains all but one murine ICR in vivo. Furthermore, the early embryonic expression of human ZNF445 and its intolerance to loss of function mutations in the population suggest a critical role in human development.
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| |
|
| Overall design |
Study of ZFP445 ChIP-seq in different mouse genomic backgrounds and ZFP57 and KAP1 ChIP-seq in human. All with their respective inputs.
|
| Web link |
http://genesdev.cshlp.org/content/33/1-2/49.long
|
| |
|
| Contributor(s) |
Coluccio A, Shi H, Duc J |
| Citation(s) |
30602440 |
| |
| Submission date |
Jun 05, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Julien Duc |
| E-mail(s) |
julien.duc@epfl.ch
|
| Organization name |
EPFL
|
| Department |
School of Life Science
|
| Lab |
LVG
|
| Street address |
Station 19 CH-1015 Lausanne
|
| City |
Lausanne |
| State/province |
VAUD |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (5)
|
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA474751 |
| SRA |
SRP149843 |
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