|
| Status |
Public on Jul 04, 2018 |
| Title |
Comprehensive RNA-Seq profiling in PBMCs of ALS patients and healthy controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Coding and long non-coding RNA metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from Sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1, C9Orf72 and VCP genes) and healthy controls. Our aim is the whole-transcriptome characterization of PBMCs content, both in terms of coding and non coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Out dataset is a starting point for the omni-comprehensive analysis of coding and long non coding RNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.
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| |
|
| Overall design |
whole transcriptome RNA-Seq profiling in PBMCs of ALS and healthy donors
|
| |
|
| Contributor(s) |
Zucca S, Gagliardi S, Pandini C, Diamanti L, Bordoni M, Sproviero D, Arigoni M, Olivero M, Pansarasa O, Ceroni M, Calogero RA, Cereda C |
| Citation(s) |
30720798 |
| |
| Submission date |
Jun 03, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Raffaele A Calogero |
| E-mail(s) |
raffaele.calogero@unito.it
|
| Phone |
++39 0116706454
|
| Organization name |
University of Torino
|
| Department |
Molecular Biotechnology Center
|
| Lab |
Bioinformatics and Genomics Unit
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
To |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE115262 |
RNA-Seq profiling in PBMCs and spinal cord from ALS patients and healthy controls |
|
| Relations |
| BioProject |
PRJNA474387 |
| SRA |
SRP149638 |
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