|
| Status |
Public on Jun 11, 2018 |
| Title |
Gene expression changes in Ing1- and Gadd45a- single- or double-knockout mouse embryonic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We previously found that ING1b is required to target GADD45a-mediated active DNA-demethylation via TET1 to specific loci. In order to study the impact of ING1-GADD45a on gene expression, we compared the expression profile of wildtype mouse embryonic fibroblasts (MEFs) with Ing1- and Gadd45a- single- or double-knockout (DKO) MEFs.
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| |
|
| Overall design |
Gene expression profiling in all 4 genotypes of undifferentiated MEFs in triplicates.
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| |
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| Contributor(s) |
Schäfer A, Mekker B, Mallick M, Vastolo V, Karaulanov E, Sebastian D, von der Lippen C, Epe B, Downes DJ, Scholz C, Niehrs C |
| Citation(s) |
29884649 |
| |
| Submission date |
May 31, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Emil Karaulanov |
| Organization name |
Institute of Molecular Biology
|
| Lab |
Bioinformatics Core Facility
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
| GSM3169047 |
MEF_undifferentiated_RNASeq_Gadd45a-KO_rep1 |
| GSM3169048 |
MEF_undifferentiated_RNASeq_Gadd45a-KO_rep2 |
| GSM3169049 |
MEF_undifferentiated_RNASeq_Gadd45a-KO_rep3 |
| GSM3169050 |
MEF_undifferentiated_RNASeq_Ing1-KO_rep1 |
| GSM3169051 |
MEF_undifferentiated_RNASeq_Ing1-KO_rep2 |
| GSM3169052 |
MEF_undifferentiated_RNASeq_Ing1-KO_rep3 |
| GSM3169053 |
MEF_undifferentiated_RNASeq_DKO_rep1 |
| GSM3169054 |
MEF_undifferentiated_RNASeq_DKO_rep2 |
| GSM3169055 |
MEF_undifferentiated_RNASeq_DKO_rep3 |
|
| This SubSeries is part of SuperSeries: |
| GSE99607 |
Impaired DNA demethylation of C/EBP sites causes premature aging |
|
| Relations |
| BioProject |
PRJNA474036 |
| SRA |
SRP149486 |
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