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Series GSE114952 Query DataSets for GSE114952
Status Public on Jun 24, 2018
Title Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary We profile 36,199 single-cell transcriptomes at multiple timepoints throughout a highly efficient chemical reprogramming system using RNA-sequencing and reconstruct their progression trajectories. Through identifying sequential molecular events, we reveal that the dynamic early embryonic-like programs account for successful reprogramming from XEN-like state to pluripotency, including the transcriptomic signature of concomitant two-cell (2C) embryonic-like and early pluripotency program and the epigenetic signature of notable genome-wide DNA demethylation.
Overall design Single cell RNA-Seq, bulk RNA-Seq and whole-genome bisulfite sequencing (WGBS) analyse the molecular mechanism of highly efficient chemical reprogramming system.
Contributor(s) Zhao T, Fu Y, Zhu J, Liu Y, Zhang Q, Deng H
Citation Ting Zhao, Yao Fu, Jialiang Zhu, Yifang Liu, Qian Zhang, Zexuan Yi, Shi Chen, Zhonggang Jiao, Xiaochan Xu, Junquan Xu, Shuguang Duo, Yun Bai, Chao Tang, Cheng Li, Hongkui Deng. Single-Cell RNA-Seq Reveals Dynamic Early Embryonic-like Programs during Chemical Reprogramming. Cell Stem Cell 2018;23:1-15. doi:10.1016/j.stem.2018.05.025
Submission date May 27, 2018
Last update date Mar 01, 2019
Contact name Yifang Liu
Organization name Peking University
Street address Haidian District
City Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (30)
GSM3160734 scRNA-Seq_MEF, XEN-like cells and CiPSCs
GSM3160735 scRNA-Seq_SI D5
GSM3160736 scRNA-Seq_SI D12
BioProject PRJNA473270
SRA SRP149052

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Supplementary file Size Download File type/resource
GSE114952_AGG.tar.gz 357.0 Mb (ftp)(http) TAR
GSE114952_ESCs.tar.gz 110.9 Mb (ftp)(http) TAR
GSE114952_RAW.tar 1009.5 Mb (http)(custom) TAR (of BEDGRAPH)
GSE114952_RNA-Seq_FPKM.xlsx 4.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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