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| Status |
Public on May 25, 2020 |
| Title |
RNA-Seq of sorted E10.5 and E11.5 AGM cell populations from Gata3-GFP embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of this study was to examine the whole transcriptome of pools of 20 cells of Gata3-expressing AGM subpopulations (haematopoietic [HC], mesenchymal [MC], sympathetic nervous system [SNS], and endothelial [EC]) and compare them to their non-Gata3 expressing counterparts
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| Overall design |
Cells were sorted into: Gata3+ EC: GFP+ Cdh5+ CD41- CD45-, Gata3- EC: GFP- Cdh5+ CD41- CD45-, Gata3+ HC: GFP+ CD41+ CD45+, Gata3- HC: GFP- CD41+ CD45+, Gata3+ MC: GFP+ Pdgfrb+, Gata3- MC: GFP- Pdgfrb+, Gata3+ SNS: GFP+ Ngfr+ and Gata3- SNS: GFP- Ngfr+. Each population was then sorted into the lysis buffer in a 96-well plate at 20 cells per well.
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| Contributor(s) |
Zaidan N, Diamanti E, Ottersbach K |
| Citation(s) |
35905741 |
| |
| Submission date |
May 25, 2018 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Evangelia Diamanti |
| E-mail(s) |
ed347@cam.ac.uk
|
| Phone |
01223 62317
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| Organization name |
University of Cambridge
|
| Department |
Haematology
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| Lab |
Gottgens
|
| Street address |
Wellcome Trust / MRC Building, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (160)
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| Relations |
| BioProject |
PRJNA473139 |
| SRA |
SRP148979 |
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