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| Status |
Public on May 26, 2020 |
| Title |
Prediabetes Induced by a Single Autoimmune B cell Clone [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
SCNT-derived B cell model displays spontaneous germinal center reaction, infiltration of pancreas (in RAG1-/- background) and impaired glucose tolerance.
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| Overall design |
To better understand the role of B cells in autoimmune diabetes, we used somatic cell nuclear transfer (SCNT) to develop a novel model from a physiological pancreas-infiltrating B cell on pure non-obese diabetic (NOD) background. Contrary to previous transgenic autoimmune B cell models, the SCNT-derived B cell model (referred to as B1411) displayed neither a developmental block nor anergy. Instead, B1411 underwent spontaneous germinal center reaction, and on RAG1-deficient background B1411 was capable of infiltrating the pancreas, and of undergoing class-switch recombination. RNA-Seq analysis identified 93 differentially expressed genes in B1411, among which were Irf7, Usp18, Mda5 that had been linked to a potential viral etiology of autoimmune diabetes, as well as various members of the oligoadenylate synthase (OAS) family. Strikingly, when challenged with glucose B1411-Rag1-/- mice displayed impaired glucose tolerance.
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| Contributor(s) |
Ke E, Kirak O, Ku M |
| Citation(s) |
32625203 |
| |
| Submission date |
May 23, 2018 |
| Last update date |
Aug 25, 2020 |
| Contact name |
Manching Ku |
| Organization name |
Medical Center - University of Freiburg
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| Department |
Hematology and Oncology
|
| Street address |
Mathildenstrasse 1
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE114831 |
Prediabetes Induced by a Single Autoimmune B cell Clone |
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| Relations |
| BioProject |
PRJNA472781 |
| SRA |
SRP148795 |
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