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| Status |
Public on Jul 18, 2018 |
| Title |
Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A difference in the transcriptomes of 8F10 T cells sourced from the NOD (8F10-NOD) or B16A (8F10-B16A) hosts was analyzed. As a result we found a number of biological pathways upregulated in the 8F10-NOD T cells including oxidative phosphorylation (OXPHOS), Myc targets, fatty acid metabolism, mTOR complex 1 (mTORC1) signaling.
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| Overall design |
T and B cell-depleted bone marrow stem cells from TCR alpha chain-deficient insulin peptide-specific 8F10 mice (CD45.2) were adoptively transferred into non-lethally irradiated NOD or B16A hosts (CD45.1). After 6 weeks of parking, the 8F10 T cells were FACS-sorted from inguinal lymph nodes of either host and subjected to RNA sequencing.
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| Contributor(s) |
Zakharov P, Wan XN, Carrero JA |
| Citation(s) |
30022165 |
| |
| Submission date |
May 23, 2018 |
| Last update date |
Nov 09, 2020 |
| Contact name |
Pavel Zakharov |
| E-mail(s) |
pavel.n.zaharov@gmail.com
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| Organization name |
Wash.Univ in St. Louis
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| Department |
Pathology and Immunology
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| Street address |
660 S Euclid Ave Box 8118
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA472771 |
| SRA |
SRP148788 |
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