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| Status |
Public on May 23, 2018 |
| Title |
Regulation of mRNA half-life by an inhibitor of human decapping enzyme Dcp2 following transcription shutoff in HEK293T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the application of next generation sequencing for measurement of mRNA levels under the effect of a macrocyclic inhibtor of human decapping enzyme 2 (hDcp2).
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| |
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| Overall design |
Examination of mRNA levels 0 h and 2 h after transcription block by actinomycin D in HEK293T cells treated by either 2 μM macrocyclic inhibitor CP25 or the same volume of vehicle for 18 h in triplicate.
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| |
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| Contributor(s) |
Luo Y, Slavoff S |
| Citation missing |
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| |
| Submission date |
May 22, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Sarah Slavoff |
| E-mail(s) |
sarah.slavoff@yale.edu
|
| Organization name |
Yale University
|
| Department |
Chemistry
|
| Street address |
300 Heffernan Dr
|
| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA472608 |
| SRA |
SRP148695 |
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