|
| Status |
Public on May 09, 2019 |
| Title |
Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior II |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Astrocytes tile the central nervous system, but their functions in neural microcircuits in vivo and their roles in mammalian behavior remain incompletely defined. We used 2-photon laser scanning microscopy (2PLSM), electrophysiology, MINIscopes, RNA-seq and a new genetic approach to characterize the effects of reduced striatal astrocyte Ca2+ signaling in vivo. In wild type mice, reducing striatal astrocyte Ca2+-dependent signaling increased repetitive self-grooming behaviors by altering medium spiny neuron (MSN) activity. The mechanism involved astrocyte-mediated neuromodulation mediated by ambient GABA and was corrected by blocking astrocyte GABA transporter 3 (GAT-3). Furthermore, in a mouse model of Huntington’s disease, dysregulation of GABA and astrocyte Ca2+ signaling accompanied excessive self-grooming, which was relieved by blocking GAT-3. Assessments with RNA-seq revealed astrocyte genes and pathways regulated by Ca2+ signaling in a cell autonomous and non-cell autonomous manner, including Rab11a, a regulator of GAT-3 functional expression. Thus, striatal astrocytes contribute to neuromodulation controlling obsessive-compulsive-like behavior in mice.
|
| |
|
| Overall design |
Total 16 samples were used in this data set. Whole striata were extraced from 8 adult Aldh1l1-Cre/ERT2 x Ribotag mice: 4 mice received AAV encoding a control protein and 4 mice received AAV encoding a protein that suppresses calcium signals named hPMCA2w/b. After homogenization, RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag.
|
| |
|
| Contributor(s) |
Yu X, Taylor AW, Nagai J, Golshani P, Evans CJ, Coppola G, Khakh BS |
| Citation(s) |
30174118 |
| |
| Submission date |
May 22, 2018 |
| Last update date |
May 09, 2019 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE114757 |
Reducing astrocyte calcium signaling in vivo alters striatal microcircuits and causes repetitive behavior |
|
| Relations |
| BioProject |
PRJNA472569 |
| SRA |
SRP148645 |
Less...
More...