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| Status |
Public on May 07, 2019 |
| Title |
Ribosome profilling and RNA Sequencing of WT and Rasgrp1Anaef mouse CD4+ T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine what mRNAs are translated at baseline in CD4+ T cells, and whether these are altered in Rasgrp1Anaef CD4+ T cells, which have a point mutation in the Ras GEF Rasgrp1, before the onset of autoimmunity, we performed sequencing of ribosome-protected fragments as well as total RNA from WT and Anaef T cells. We identified a number of target genes that are translated at baseline, many of which have been shown to be downstream of the kinase mTOR.
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| Overall design |
CD4+ T cells from the lymph nodes of mice that are WT/WT or Rasgrp1Anaef/Rasgrp1Anaef were purified by a negative isolation magnetic bead-based kit. 20000 cells were lysed and total RNA was isolated (4 samples per genotpye) and RNA was sequenced (Illumina HiSeq 4000); from the rest of the cells in the pool. samples were pooled 2 by 2 (for 2 total samples per genotype), lysed, and ribosome-associated mRNAs were isolated and sequenced (Illumina HiSeq 4000)
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| Contributor(s) |
Myers D, Roose J |
| Citation(s) |
31067469 |
| |
| Submission date |
May 22, 2018 |
| Last update date |
Aug 06, 2019 |
| Contact name |
Darienne Ross Myers |
| E-mail(s) |
dariennemyers@gmail.com
|
| Phone |
7738525995
|
| Organization name |
UCSF
|
| Department |
Anatomy
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| Lab |
Jeroen Roose
|
| Street address |
513 Parnassus Ave, HSW 1330A, Box 0452
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA472693 |
| SRA |
SRP148758 |
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