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| Status |
Public on Aug 29, 2018 |
| Title |
RNA-seq of the lacrimal gland epithelial tissue in control vs Pea3-depleted tissue in mouse embyro |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of the study is to find the transcriptional targets downstream of Pea3-mediated signaling in the lacrimal gland epithelium. In order to look for the potential targets, we performed laser capture microdissection of the lacrimal gland epithelial tissue at embryonic day E14.5 from control embryos as well as mutant embryos containing epithelium-specific deletion of Pea3. After tissue harvest, RNA was extracted. Conversion to cDNA as well as amplification was performed by Clontech SMART-seq v4 Ultra low input RNA kit, as well as cDNA library construction was performed using Nextera XT DNA library preparation kit by core facility at Columbia university prior to RNA sequencing. After preparation of cDNA library, each sample was sequenced using Illumina platform.
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| Overall design |
For this experiment, 3 control samples and 3 mutant samples were analysed.
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| |
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| Contributor(s) |
Garg A, Zhang X |
| Citation(s) |
30188892 |
| |
| Submission date |
May 16, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Xin Zhang |
| E-mail(s) |
xz2369@cumc.columbia.edu
|
| Phone |
2123424446
|
| Organization name |
Columbia University
|
| Department |
Ophthalmology
|
| Street address |
635 W. 165th St.
|
| City |
New york |
| State/province |
New york |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA471632 |
| SRA |
SRP147008 |
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