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| Status |
Public on Jun 01, 2018 |
| Title |
Chromatin accessibility dynamics across C. elegans development and ageing [scap] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
An essential step for understanding the transcriptional circuits that control development and physiology is the global identification and characterization of regulatory elements. Here we present the first map of regulatory elements across the development and ageing of an animal, identifying 42,245 elements accessible in at least one C. elegans stage. Based on nuclear transcription profiles, we define 15,918 protein-coding promoters and 17,918 putative enhancers, and find that both types of element can drive orientation-independent transcription. Additionally, hundreds of promoters produce transcripts antisense to protein coding genes, suggesting involvement in a widespread regulatory mechanism. We find that the accessibility of most elements is regulated during development and/or ageing and that patterns of accessibility change are linked to specific developmental or physiological processes. The map and characterization of regulatory elements across C. elegans life provides a platform for understanding how transcription controls development and ageing.
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| Overall design |
Short capped chromatin associated RNA-seq was performed to monitor transcription initiation in wild-type C. elegans at six developmental stages
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| Contributor(s) |
Ahringer J, Jänes J |
| Citation(s) |
30362940 |
| |
| Submission date |
May 15, 2018 |
| Last update date |
Nov 29, 2018 |
| Contact name |
Julie Ahringer |
| E-mail(s) |
ja219@cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
The Gurdon Institute
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| Street address |
Tennis Court Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18730 |
Illumina HiSeq 1500 (Caenorhabditis elegans) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE114494 |
Chromatin accessibility dynamics across C. elegans development and ageing |
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| Relations |
| BioProject |
PRJNA471536 |
| SRA |
SRP146247 |
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