|
Status |
Public on Apr 13, 2021 |
Title |
Isolation and characterization of hESC-derived heart field-specific cardiomyocytes unravels new insights into their transcriptional and electrophysiological profiles |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
We prospectively isolate and characterize first and second heart field- and nodal-like cardiomyocytes using a double reporter line from human embryonic stem cells
|
|
|
Overall design |
We performed RNA sequencing of hESC derived CMs at various timepoints, and scRNA seq on Day 20 hESC derived CMs and human fetal heart at 10 weeks of gestation.
|
|
|
Contributor(s) |
Pezhouman A, Engel JL, Nguyen NB, Skelton RP, Gilmore WB, Qiao R, Sahoo D, Zhao P, Elliott DA, Reza A |
Citation(s) |
33744937 |
|
Submission date |
May 13, 2018 |
Last update date |
Apr 22, 2021 |
Contact name |
Reza Ardehali |
Organization name |
UCLA
|
Department |
Cardiology
|
Street address |
675 Charles E Young dr
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platforms (3) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
Samples (25)
|
|
Relations |
BioProject |
PRJNA471193 |
SRA |
SRP145551 |
Supplementary file |
Size |
Download |
File type/resource |
GSE114373_FHF_barcodes.tsv.gz |
103.1 Kb |
(ftp)(http) |
TSV |
GSE114373_FHF_features.tsv.gz |
297.6 Kb |
(ftp)(http) |
TSV |
GSE114373_FHF_matrix.mtx.gz |
116.5 Mb |
(ftp)(http) |
MTX |
GSE114373_SHF_barcodes.tsv.gz |
112.4 Kb |
(ftp)(http) |
TSV |
GSE114373_SHF_features.tsv.gz |
297.6 Kb |
(ftp)(http) |
TSV |
GSE114373_SHF_matrix.mtx.gz |
145.0 Mb |
(ftp)(http) |
MTX |
GSE114373_hFetal_10w_LV_barcodes.tsv.gz |
45.3 Kb |
(ftp)(http) |
TSV |
GSE114373_hFetal_10w_LV_features.tsv.gz |
297.6 Kb |
(ftp)(http) |
TSV |
GSE114373_hFetal_10w_LV_matrix.mtx.gz |
49.1 Mb |
(ftp)(http) |
MTX |
GSE114373_hFetal_10w_RV_barcodes.tsv.gz |
46.4 Kb |
(ftp)(http) |
TSV |
GSE114373_hFetal_10w_RV_features.tsv.gz |
297.6 Kb |
(ftp)(http) |
TSV |
GSE114373_hFetal_10w_RV_matrix.mtx.gz |
43.0 Mb |
(ftp)(http) |
MTX |
GSE114373_reza-2019-tpm-expr.txt.gz |
3.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |