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| Status |
Public on Oct 02, 2018 |
| Title |
A comprehensive spatial-temporal transcriptomic analysis of differentiating nascent mouse lens epithelial and fiber cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The lens is comprised of the anterior lens epithelium and posterior lens fibers, which form the bulk of the lens. The RNAseq data enables identification of lens epithelium and fiber differentially expressed genes and temporally differentially expressed genes which were also validated by qRTPCR. The present RNA-seq data serves as a comprehensive reference resource for deciphering molecular principles of normal mammalian lens differentiation, mapping a full spectrum of signaling pathways and DNA-binding transcription factors operating in both lens compartments, and predicting novel pathways required to establish lens transparency.
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| Overall design |
The lens epithelium and fiber were collected from E14.5, E16.5, E18.5 CD1 mouse embryos and P0.5 mice. Three biological replicates were prepared. The RNA were used for total RNAseq library construction and sent for 100bp paired end sequencing on illumina Hiseq 2500. The genes detected by RNAseq were then validated by qRTPCR.
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| Contributor(s) |
Zhao Y, Zheng D, Cvekl A |
| Citation(s) |
29883638, 38643244 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 EY014237 |
Transcriptional Control of the Mouse aA-crystallin locus |
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC. |
Ales Cvekl |
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| |
| Submission date |
May 01, 2018 |
| Last update date |
May 17, 2024 |
| Contact name |
Ales Cvekl |
| E-mail(s) |
ales.cvekl@einstein.yu.edu
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| Organization name |
Albert Einstein College of Medicine
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| Department |
The Departments Ophthalmology and Visual Sciences, Genetics
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| Lab |
Dr. Cvekl's lab
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| Street address |
1300 Morris Park Avenue, Ullman 123
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| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA454369 |
| SRA |
SRP144153 |
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