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| Status |
Public on Apr 22, 2019 |
| Title |
A lineage commitment checkpoint in early ILC development [bulk RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Understanding how cellular function is imprinted during development requires the identification of factors controlling lineage specification and commitment, and the intermediate progenitors in which they act. Using population level and single cell approaches, we examine transcriptional and functional heterogeneity within early innate lymphoid cells (ILC) progenitors. We identify a developmental bifurcation toward dendritic cell fate that reveals the uncommitted state of early specified ILC progenitors. We subsequently characterize an ILC-commitment checkpoint controlled by the transcription factor TCF-1. The present study reveals unexpected heterogeneity within early innate progenitor populations, and characterizes lineage infidelity that accompanies early ILC specification prior to commitment.
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| Overall design |
transcriptional profiling of early ILC progenitors (EILP, ILCP), and common lymphoid progenitors (ALP) from WT or Tcf7 null mice was performed by RNA sequencing
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| |
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| Contributor(s) |
Harly C, Cam M, Kenney D, Raabe T, Yang Q, Xue H, bhandoola A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 27, 2018 |
| Last update date |
Apr 24, 2019 |
| Contact name |
Margaret C Cam |
| E-mail(s) |
maggie.cam@nih.gov
|
| Phone |
240-760-7179
|
| Organization name |
NIH
|
| Street address |
Bldg 37/3041C
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE113767 |
A lineage commitment checkpoint in early ILC development |
|
| Relations |
| BioProject |
PRJNA453957 |
| SRA |
SRP143470 |
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