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| Status |
Public on May 10, 2008 |
| Title |
Rosiglitazone Treatment Reduces Diabetic Neuropathy in STZ treated DBA/2J mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Diabetic Neuropathy (DN) is a common complication of diabetes. Currently, there is no drug treatment to prevent or slow the development of DN. Rosiglitazone (Rosi) is a potent insulin sensitizer and may also slow the development of DN by a mechanism independent of its effect on hyperglycemia. A two by two design was used to test the effect of Rosi treatment on the development of DN. Streptozotocin-induced diabetic DBA/2J mice were treated with Rosi. DN and oxidative stress were quantified, and gene expression was profiled using the Affymetrix Mouse Genome 430 2.0 microarray platform. An informatics approach identified key regulatory elements activated by Rosi. Diabetic DBA/2J mice developed severe hyperglycemia, DN and elevated oxidative stress. Rosi treatment did not affect hyperglycemia but did reduce oxidative stress and prevented development of thermal hypoalgesia. Two novel transcription factor binding modules were identified that may control genes correlated to changes in DN following Rosi treatment: SP1F_ZBPF and EGRF_EGRF. Rosi treatment reduced oxidative stress and DN independent of its insulin sensitizing effects. Gene expression profiling identified two novel targets activated by Rosi treatment. These targets may be useful in designing drugs with the same efficacy as Rosi in treating DN but with fewer undesirable effects.
Keywords: disease and treatment analysis
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| |
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| Overall design |
There were 4 groups:
Control
Control + Rosi
Diabetic (Type 1)
Diabetic + Rosi
Affymetrix chips were run on five mice from each group. One chip (in the Control group) failed quality control measures and was excluded.
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| |
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| Contributor(s) |
Wiggin TD, Kretzler M, Pennathur S, Sullivan KA, Brosius FC, Feldman EL |
| Citation(s) |
18583417 |
| |
| Submission date |
May 05, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Timothy David Wiggin |
| E-mail(s) |
twiggin@med.umich.edu
|
| URL |
http://www.med.umich.edu/pnrd/
|
| Organization name |
University of Michigan
|
| Department |
Neurology
|
| Street address |
109 Zina Pitcher Pl., Rm 5368
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-2200 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (19)
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| Relations |
| BioProject |
PRJNA106555 |
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