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| Status |
Public on Apr 20, 2019 |
| Title |
Bulk RNA sequencing of VE-Cadherin+GFP+, VE-Cadherin- GFP+ and VE-Cadherin+GFP- populations from E11.5 miR144/451+/GFP mouse yolk sac. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of the experiment was to compare a newly defined population VE-Cadherin+GFP+ to control populations, VE-Cadherin- GFP+ and VE-Cadherin+GFP-.
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| Overall design |
Yolk sacs from E11.5 miR144/451+/GFP mouse embryos were isolated and single cell suspensions were generated. Cells were stained with the anti-VE-Cadherin antibody and 24 VE-Cadherin+GFP+ cells (n=2), 25 VE-Cadherin- GFP+ cells (n=3) and 25 VE-Cadherin+GFP- cells (n=3) were sorted by flow cytometry. The SMART-Seq 2 method was then used to produce cDNA from these eight samples. Sequencing libraries were generated and were later sequenced together on the Illumina Next Seq 500 sequencer.
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| Contributor(s) |
Lancrin C, Ganter K |
| Citation missing |
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| Submission date |
Apr 19, 2018 |
| Last update date |
Apr 20, 2019 |
| Contact name |
Christophe Lancrin |
| E-mail(s) |
christophe.lancrin@embl.it
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| Organization name |
EMBL
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| Department |
EMBL Rome
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| Street address |
Via Ramarini 32
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| City |
Monterotondo |
| State/province |
RM |
| ZIP/Postal code |
00015 |
| Country |
Italy |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA451028 |
| SRA |
SRP141223 |
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