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| Status |
Public on Oct 18, 2018 |
| Title |
Mechanosensitive ion channel regulates tissue stiffening to promote glioma aggression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Tissue stiffening is a physical hallmark of solid tumor. In our study, we found that the mechanosensitive ion channel PIEZO1 can be tumor cells force sensor and transducer that regulate the altered tissue mechanics. We examined the transcriptional changes modulated by depletion of PIEZO1 in two human GBM cell lines. We found that with PIEZO1 KD, the components involved in ECM organization, focal adhesion formation, cytoskeleton, and mitosis, which contribute to tissue stiffness, are down-regulated.
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| Overall design |
RNA-Seq of G508 and G532 human GBM cell lines treated with scrambled shRNA, PIEZO1 shRNA #1, PIEZO1 shRNA #2, in triplicates.
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| Contributor(s) |
Chen X, Yin W |
| Citation(s) |
30344046 |
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| Submission date |
Apr 17, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Xi Huang |
| E-mail(s) |
xi.huang@sickkids.ca
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| Organization name |
SickKids
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| Street address |
686 Bay Street,
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| City |
Toronto |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA450606 |
| SRA |
SRP140640 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE113261_RAW.tar |
41.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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