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Series GSE113166 Query DataSets for GSE113166
Status Public on May 11, 2018
Title Genome-wide expression microarray data of MCSP- and ABCB5-enriched CTCs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Circulating Tumour Cells (CTCs) act as tumour seeds for haematogeneous melanoma spread of melanoma. Since CTCs have been reported to be heterogeneous in melanoma, the characterisation of the different melanoma CTC subpopulations harbours biological and clinical significance. For detection of melanoma CTCs, melanoma-associated markers (i.e. MCSP, melanoma-associated chondroitin sulphate proteoglycan) have been used; however, recent studies have found that melanoma CTCs commonly express ABCB5 (ATP-binding cassette sub-family B member 5) a melanoma-initiating marker. Here, we used a genome-wide expression microarray to interrogate the transcriptome of MCSP-enriched and ABCB5-enriched CTC fraction in order to provide a better understanding of their biology and role in melanoma metastasis. Findings from this study highlited the distinct transcriptional programming of both CTC subpopulations.
 
Overall design Blood samples from healthy donors (n=6) and metastatic melanoma patients (n=6) were incubated with magnetic beads coated with antibodies against MCSP or ABCB5 for immunomagnetic enrichment of either MCSP or ABCB5 CTCs. Total RNA was extracted from the total of 24 samples and microarray gene expression profiling was performed uisng the Affymetrix® GeneChip® genome 2.0 ST array protocol (Affymetrix, US). Gene expression data from MCSP or ABCB5 enriched CTCs of healthy donors and melanoma patient blood samples were compared.
 
Contributor(s) Aya-Bonilla CA, Gray ES, Ziman M
Citation(s) 30769764
Submission date Apr 16, 2018
Last update date Sep 01, 2022
Contact name Carlos A Aya-bonilla
E-mail(s) carlosaya2@yahoo.com.ar, carlos.aya-bonilla@telethonkids.org.au
Organization name Telethon Kids Institute
Street address 32 kinship street
City perth
State/province WA
ZIP/Postal code 6155
Country Australia
 
Platforms (1)
GPL17692 [HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array [transcript (gene) version]
Samples (24)
GSM3098379 MCSP-enriched Healthy Donor blood sample (E07_MZ_M)
GSM3098380 MCSP-enriched Healthy Donor blood sample (F07_RA_M)
GSM3098381 MCSP-enriched Healthy Donor blood sample (G07_DM_M)
Relations
BioProject PRJNA450337

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE113166_RAW.tar 103.3 Mb (http)(custom) TAR (of CEL)
GSE113166_fold-change.txt.gz 48.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record
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