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| Status |
Public on Apr 16, 2018 |
| Title |
LH3 overexpression reverses profound alterations of gene transcriptional profile in cerebral vessels in hypertension-induced spontaneous intracerebral hemorrhage |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To gain mechanistic insights into the protection effects of lysyl hydroxylase (LH3) on reducing the susceptibility to intracerebral hemorrhage(ICH), a whole transcriptomic analysis of cerebral vessels were performed using RNA-seq. It is noteworthy that our preliminary data demonstrated that mice did not developed ICH until day 7 after AngII plus L-NAME infusion, cerebral vessels for RNA-seq were acquired at day 5 in order to provide the mechanistic or causal evidence of a direct participatory role of LH3 to the protection effects of ICH.
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| Overall design |
15 samples. There are 3 groups: GFP-Veh group, mice were stereotactic injection of AAV-GFP and treated with saline. GFP-AL group, mice were stereotactic injection of AAV-GFP and treated with AngII plus L-NAME. LH3-AL group, mice were stereotactic injection of AAV-LH3 and treated with AngII plus L-NAME. Mice of each group were transcardially perfused with ice-cold phosphate-buffered saline, brain were collected and cerebral vessels were isolated.
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| Contributor(s) |
Li H, Xu H |
| Citation missing |
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| Submission date |
Apr 15, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
li Michael |
| E-mail(s) |
lizz007sci@126.com
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| Organization name |
Basepedia
|
| Street address |
beiqing road
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| City |
beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA450267 |
| SRA |
SRP140161 |
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