 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 24, 2018 |
| Title |
RNA-seq profiling of human thymic Tregs cultured in conditions containing Th1-cytokines, Retinoic acid or control conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine the effects of Th1-cytokines (IL-12 + IFN-gamma) or retinoic acid (RA) on expanding human Tregs isolated from pediatric thymus. Cells were stimulated after isolation and cultured for 13 days before RNAsequencing was performed.
|
| |
|
| Overall design |
Human Tregs from pediatric thymus were isolated, stimulated via CD3/CD28 and expanded in vitro for 7 days (with IL-2 and rapamycin), followed by CD3/CD28 restimulation and culture (with IL-2) until day 13. Cells were polarized to become Th1-Tregs by addition of IL-12 and IFN-gamma from day 0 to 7 of culture followed by neutral conditions from day 7 to 13 (Th1 -> 0) or from day 0 to 13 of culture (Th1 -> Th1). Retinoic acid was added from day 7 to 13 of culture (0 -> RA) to induce a gut-homing phenotype. Tregs without Th1-cytokines or RA served as controls (0 -> 0). Samples are from 3 subjects (PXX8, T016, T018) and each sample was used for all conditions (paired-design).
|
| |
|
| Contributor(s) |
Levings MK, Hoeppli RE |
| Citation(s) |
29766641 |
| |
| Submission date |
Apr 10, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Megan K Levings |
| E-mail(s) |
megan.levings@ubc.ca
|
| Phone |
604-875-2000
|
| Organization name |
University of British Columbia
|
| Street address |
950 West 28th Ave
|
| City |
Vancouver |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA449524 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112923_Th1vsRAvsNeutralTreg_ProcessedCounts.xlsx |
1.9 Mb |
(ftp)(http) |
XLSX |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...