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| Status |
Public on Oct 08, 2018 |
| Title |
nanoPARE: Parallel analysis of RNA 5' ends from low input RNA |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A diversity of RNA molecule 5' ends are generated during transcriptional and post-transcriptional processes. Different RNA ends can confer or represent different functional activities and thus the identification of RNA end usage dynamics contributes to the functional characterization of RNA molecules. Here we present a method that enables the accurate identification of RNA 5' ends from samples with low amounts of total RNAs, and thus allow characterization of RNA regulatory mechanisms in specific cell-types.
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| Overall design |
NanoPARE, or RNA 5' end enrichment, libraries were constructed for three biological replicates of ten conditions/genotypes. mRNA-Seq libraries were generated were constructed for three biological replicates of two conditions/genotypes.
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| Contributor(s) |
Nodine M, Schon M, Kellner M |
| Citation(s) |
30355603, 35768836 |
| |
| Submission date |
Apr 09, 2018 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Michael D Nodine |
| E-mail(s) |
michael.nodine@wur.nl
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| Organization name |
Wageningen University & Research
|
| Department |
Plant Sciences
|
| Lab |
Molecular Biology
|
| Street address |
Radix West
|
| City |
Wageningen |
| State/province |
Gelderland |
| ZIP/Postal code |
6708 PB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA449355 |
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