NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE112860 Query DataSets for GSE112860
Status Public on Jun 08, 2018
Title mRNA expression of murine hematopoietic stem cells and ex vivo murine MLL-AF9 cells deficient for total PRDM16 or f-PRDM16, and AF9 cells overexpressing individual PRDM16 isoforms
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Group 1 -- WT or PRDM16-KO ex vivo murine MLL-AF9 cells, and PRDM16-KO AF9 cells overexpressing either f-PRDM16 or s-PRDM16. Group 2 -- WT or total PRDM16-KO murine HSCs isolated from adult BM. Group 3 -- WT or total PRDM16-KO murine HSCs isolated from fetal liver. Group 4 -- WT or f-PRDM16-KO murine HSCs (expressing s-PRDM16 only) isolated from fetal liver.
 
Overall design Sorted ex vivo MLL-AF9 cell (group 1) or sorted murine HSC (groups 2-4) mRNA profiles were generated by deep sequencing, in 3 or 4 independent replicates, using Illumina HiSeq2500/NS500
 
Contributor(s) Snoeck H, Corrigan D, Strikoudis A
Citation(s) 29878897
Submission date Apr 09, 2018
Last update date Mar 25, 2019
Contact name Hans-Willem Snoeck
E-mail(s) hs2680@cumc.columbia.edu
Organization name Columbia University Medical Center
Department Medicine
Street address 650 W. 168th Street, BB816
City New York
State/province NY
ZIP/Postal code 10032
Country USA
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (32)
GSM3090075 Ex_vivo_AF9_cells_WT-PRDM16+EV_replicate_1
GSM3090076 Ex_vivo_AF9_cells_WT-PRDM16+EV_replicate_2
GSM3090077 Ex_vivo_AF9_cells_WT-PRDM16+EV_replicate_3
Relations
BioProject PRJNA449343

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112860_47bpFL_RNAseq_Counts.txt.gz 264.4 Kb (ftp)(http) TXT
GSE112860_AF9_RNAseq_Counts.txt.gz 405.3 Kb (ftp)(http) TXT
GSE112860_VAVBM_RNAseq_Counts.txt.gz 323.5 Kb (ftp)(http) TXT
GSE112860_VAVFL_RNAseq_Counts.txt.gz 290.8 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data are available on Series record
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap